Igh^tm1(Myc)Janz
Targeted Allele Detail

Summary

• Symbol: Igh^tm1(Myc)Janz
• Name: immunoglobulin heavy chain complex; targeted mutation 1, Siegfried Janz
• MGI ID: MGI:3574400
• Synonyms: c-myc-KIEmu, iMyc^Emu
• Gene: Igh Location: Chr12:113222388-115973574 bp, - strand Genetic Position: Chr12, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:96785
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Igh^tm1(Myc)Janz expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Myc (MGI:97250)

• Mutation details: A construct consisting of a 1.5 kb segment of genomic 5' flanking sequence from the mouse Myc gene with the P1/P2 promoters, the first noncoding exon, a mouse Myc intron-less cDNA clone with an in-frame 6x histidine tag sequence on the 3' end, a short segment of the 3' UTR including the major polyadenylation signal, and a loxP site flanked neomycin resistance gene cassette was inserted in opposite transcriptional orientation into into the locus 5' of the intronic enhancer Emu. The neo cassette was removed through subsequent cre-mediated recombination. This insertion of Myc mimics both the human t(8;14)(q24;q32) translocation that results in the activation of MYC in human endemic Burkitt lymphomas and the homologous mouse T(12;15) translocation that deregulates Myc in mouse plasmacytomas. (J:96785)

Disease models

Tumor Data

List all tumor models in MMHCdb carrying Igh^tm1(Myc)Janz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igh Mutation: 43 strains or lines available

References

• Original: J:96785 Park SS, et al., Insertion of c-Myc into Igh induces B-cell and plasma-cell neoplasms in mice. Cancer Res. 2005 Feb 15;65(4):1306-15
• All: 17 reference(s)