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Tg(HLA-DRB1)31Dmz
Transgene Detail
Nomenclature
Symbol: Tg(HLA-DRB1)31Dmz
Name: transgene insertion 31, Dennis M Zaller
MGI ID: MGI:3529465
Synonyms: DR4Dw4, DRB1*0401, HLA-DR4, HLA-DR4 (DRB1*0401)
Transgene: Tg(HLA-DRB1)31Dmz  Location: unknown  
Transgene
origin
Strain of Origin:  (C57BL/6J x SJL/J)F2
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
 
Tg(HLA-DRB1)31Dmz expresses 1 gene
 
Mutation detailsA chimeric MHC class II complex was created by fusing the human peptide binding domains of class II alpha and beta chains to the CD4 binding domains of murine class II alpha or beta chains. The was done by the construction of two separate transgene constructs. The alpha1 and beta1 domains isolated from human DRB1*0401 (DR4Dw4) replaced the corresponding alpha1 (aa 1-85) and beta1 (aa 1-96) domains of the H2-Ea and H2-Eb1 genes that make up mouse I-Ed. The native murine promoters were left intact. These transgene constructs were co-injected into fertilized eggs. Flow cytometry analysis indicates that virtually all B-cells and macrophages co-express the chimeric MHC class II molecules. The MHC class II chimeric molecules can be physiologically regulated as there is a 3-4 fold increase in MHC II molecules when splenic B-cells are cultured overnight with IL-4. (J:96129)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:96129 Woods A, et al., Human major histocompatibility complex class II-restricted T cell responses in transgenic mice. J Exp Med. 1994 Jul 1;180(1):173-81
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
05/12/2015
MGI 5.21
The Jackson Laboratory