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Transgene Detail
Symbol: Tg(HLA-DRB1)31Dmz
Name: transgene insertion 31, Dennis M Zaller
MGI ID: MGI:3529465
Synonyms: DR4Dw4, DRB1*0401, HLA-DR4, HLA-DR4 (DRB1*0401)
Transgene: Tg(HLA-DRB1)31Dmz  Location: unknown  
Strain of Origin:  (C57BL/6J x SJL/J)F2
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(HLA-DRB1)31Dmz expresses 1 gene
Mutation detailsA chimeric MHC class II complex was created by fusing the human peptide binding domains of class II alpha and beta chains to the CD4 binding domains of murine class II alpha or beta chains. The was done by the construction of two separate transgene constructs. The alpha1 and beta1 domains isolated from human DRB1*0401 (DR4Dw4) replaced the corresponding alpha1 (aa 1-85) and beta1 (aa 1-96) domains of the H2-Ea and H2-Eb1 genes that make up mouse I-Ed. The native murine promoters were left intact. These transgene constructs were co-injected into fertilized eggs. Flow cytometry analysis indicates that virtually all B-cells and macrophages co-express the chimeric MHC class II molecules. The MHC class II chimeric molecules can be physiologically regulated as there is a 3-4 fold increase in MHC II molecules when splenic B-cells are cultured overnight with IL-4. (J:96129)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:96129 Woods A, et al., Human major histocompatibility complex class II-restricted T cell responses in transgenic mice. J Exp Med. 1994 Jul 1;180(1):173-81
All:  16 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
The Jackson Laboratory