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Smad2tm4(SMADH3)Rob
Targeted Allele Detail
Summary
Symbol: Smad2tm4(SMADH3)Rob
Name: SMAD family member 2; targeted mutation 4, Elizabeth J Robertson
MGI ID: MGI:3527156
Synonyms: Smad2hSmad3ki
Gene: Smad2  Location: Chr18:76374651-76444034 bp, + strand  Genetic Position: Chr18, 51.42 cM
Alliance: Smad2tm4(SMADH3)Rob page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:95374
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to subsequently delete exon 2 coding sequence and insert the amino-terminal flag-tagged human SMAD3 cDNA into the locus. A triply repeated SV40 polyA sequence was inserted to ensure transcriptional termination as well as a floxed PGK-neo. Transient cre expression excised the floxed neo. Ribonuclease protection assay probes were designed to confirm expression of the human cDNA, and residual endogenous transcripts were barely detectable in mutant tissues. Recombined transcript expression levels in mutants were comparable to that of endogenous levels in wild-type animals. (J:95374)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Smad2 Mutation:  50 strains or lines available
References
Original:  J:95374 Dunn NR, et al., Mice exclusively expressing the short isoform of Smad2 develop normally and are viable and fertile. Genes Dev. 2005 Jan 1;19(1):152-63
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory