Iqgap2^tm1Vs
Targeted Allele Detail

Summary

• Symbol: Iqgap2^tm1Vs
• Name: IQ motif containing GTPase activating protein 2; targeted mutation 1, Valentina A Schmidt
• MGI ID: MGI:3526416
• Gene: Iqgap2 Location: Chr13:95763685-96028788 bp, - strand Genetic Position: Chr13, 50.26 cM, cytoband D1
• Alliance: Iqgap2^tm1Vs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:132670
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 36,241 bp genomic fragment spanning exons 18 - 31 was replaced with a PGK-neo cassette via homologous recombination. This resulted in removal of part of the IQ2 and all of the IQ3, IQ4, and GRD motifs. QRT-PCR confirmed the absence of transcript in hepatocytes and immunoblot analysis confirmed the absence of protein in liver, spleen, heart, and kidney lysates. Only in the liver was a modest increase in Iqgap1 expression detected. QRT-PCR analysis did not detect any change in F2rl2 (coding sequence contained within intron 13 of Iqgap2) expression. (J:132670)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Iqgap2 Mutation: 84 strains or lines available

References

• Original: J:132670 Schmidt VA, et al., Development of hepatocellular carcinoma in Iqgap2-deficient mice is IQGAP1 dependent. Mol Cell Biol. 2008 Mar;28(5):1489-502
• All: 4 reference(s)