Chka^Gt(XH252)Byg
Gene trapped Allele Detail

Summary

• Symbol: Chka^Gt(XH252)Byg
• Name: choline kinase alpha; gene trap XH252, BayGenomics
• MGI ID: MGI:3526395
• Synonyms: Chka^-
• Gene: Chka Location: Chr19:3901773-3944369 bp, + strand Genetic Position: Chr19, 3.57 cM
• Alliance: Chka^Gt(XH252)Byg page

Mutation origin

• Mutant Cell Line: XH252
• Germline Transmission: Earliest citation of germline transmission: J:130726
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT1Lxf Vector Type: gene trap
• Mutation details: The gene trap vector pGT1Lxf containing a beta-geo fusion was inserted into intron 5. The decrease in protein production was confirmed by western blot analysis in liver and testes extracts from heterozygous mice. Authors state that this is a null allele. (J:130726)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  XH252	CC199764 ; (MGI Seq Detail)	5' RACE	Chr19:3934471-3935936 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  12 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chka Mutation: 28 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:130726 Wu G, et al., Early embryonic lethality caused by disruption of the gene for choline kinase alpha, the first enzyme in phosphatidylcholine biosynthesis. J Biol Chem. 2008 Jan 18;283(3):1456-62
• All: 2 reference(s)