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Transgene Detail
Symbol: Tg(tetORo1-lacZ)3Conk
Name: transgene insertion 3, Bruce Conklin
MGI ID: MGI:3524978
Synonyms: TRE-lacZ
Transgene: Tg(tetORo1-lacZ)3Conk  Location: unknown  
Strain of Origin:  FVB/N
Transgene Type:    Transgenic (Inducible, Inserted expressed sequence, Reporter)
Mutation:    Insertion
Mutation detailsTwo transgenes controlled by the tetO (responsive to tet-transactivator) promoter, one containing "Ro1" and the other containing lacZ, were co-integrated so that beta-galactosidase could be used as a marker for expression. Ro1 is a prototype RASSL (Receptor Activated Solely by a Synthetic Ligand) with an N-terminal FLAG epitope that is extra-cellularly expressed and can be detected with the FLAG monoclonal antibody. Ro1 (RASSL, opioid, #1) is derived from a Kappa Opioid Receptor (G protein-coupled receptor) that was altered to reduce the activation by endogenous hormones, but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. (J:95090)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 6 anatomical structures
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
On an FVB/N genetic background, transgenic mice also carrying Tg(Myh6-tTA)6Smbf express Ro1 in the heart. Administration of the drug spiradoline results in a dramatic decrease of heart rate in these mice. X-gal staining of tissues from these mice reveals the pattern of cells that the tet system is working in. Ro1 activates Gi-signaling in response to spiradoline.
Original:  J:95090 Redfern CH, et al., Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol. 1999 Feb;17(2):165-9
All:  14 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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