Tg(tetORo1-lacZ)3Conk
Transgene Detail

Nomenclature

• Symbol: Tg(tetORo1-lacZ)3Conk
• Name: transgene insertion 3, Bruce Conklin
• MGI ID: MGI:3524978
• Synonyms: TRE-lacZ
• Transgene: Tg(tetORo1-lacZ)3Conk Location: unknown

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: Two transgenes controlled by the tetO (responsive to tet-transactivator) promoter, one containing "Ro1" and the other containing lacZ, were co-integrated so that beta-galactosidase could be used as a marker for expression. Ro1 is a prototype RASSL (Receptor Activated Solely by a Synthetic Ligand) with an N-terminal FLAG epitope that is extra-cellularly expressed and can be detected with the FLAG monoclonal antibody. Ro1 (RASSL, opioid, #1) is derived from a Kappa Opioid Receptor (G protein-coupled receptor) that was altered to reduce the activation by endogenous hormones, but preserves activation by an exogenous drug, spiradoline, allowing experimental control of G protein signaling. (J:95090)

Disease models

Expression

In Structures Affected by this Mutation:

6 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

On an FVB/N genetic background, transgenic mice also carrying Tg(Myh6-tTA)6Smbf express Ro1 in the heart. Administration of the drug spiradoline results in a dramatic decrease of heart rate in these mice. X-gal staining of tissues from these mice reveals the pattern of cells that the tet system is working in. Ro1 activates Gi-signaling in response to spiradoline.

References

• Original: J:95090 Redfern CH, et al., Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nat Biotechnol. 1999 Feb;17(2):165-9
• All: 14 reference(s)