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Targeted Allele Detail
Symbol: Slc46a2tm1Moki
Name: solute carrier family 46, member 2; targeted mutation 1, Moon Kim
MGI ID: MGI:3521803
Synonyms: TDLacZ, TSCOTLacZ
Gene: Slc46a2  Location: Chr4:59905899-59915056 bp, - strand  Genetic Position: Chr4, 32.81 cM
Germline Transmission:  Earliest citation of germline transmission: J:140121
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA 4.2-kb targeting vector consisting of IRES-LacZ with a neo-selectable marker was placed in between two BamHI sites found in the first exon of the gene locus. This vector replaced coding sequence for transmembrane domains 5 through 12. Northern blotting confirmed that the LacZ coding sequence was in a fusion transcript with the 5' portion of the gene's coding sequence. LacZ expression was detected in the thymic cortex and occasionally in the thymic corticomedullary junction. The N-terminal portion of the endogenous gene product, including transmembrane spans 1?4 of the protein, still remained expressed on the cell surface, as detected by flow cytometry. (J:140121)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 6 assay results
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Slc46a2 Mutation:  2 strains or lines available
Original:  J:140121 Ahn S, et al., TSCOT+ thymic epithelial cell-mediated sensitive CD4 tolerance by direct presentation. PLoS Biol. 2008 Aug 5;6(8):e191
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory