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Fgf2tm2Doe
Targeted Allele Detail
Nomenclature
Symbol: Fgf2tm2Doe
Name: fibroblast growth factor 2; targeted mutation 2, Thomas Doetschman
MGI ID: MGI:3512180
Synonyms: 18-kDa FGF2KO, Fgf2 lmw ko
Gene: Fgf2  Location: Chr3:37402616-37464255 bp, + strand  Genetic Position: Chr3, 18.41 cM, cytoband A2-B
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94550
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsExchange reaction of a Tag & Exchange targeting scheme 4 bp alteration without introduction of marker gene or loxP or frt sites. To produce this ko allele, the Hprt minigene in the tagged Fgf2 ko allele (Zhou et al, Nature Medicine, 4:201, 1998) was "exchanged" by gene targeting with a mutant exon 1 with the sequence CTGCAG replacing the wild-type CCATGC. The mutant sequence encodes an alanine GCA in place of the methionine ATG translational start site of the 18-kDa low molecular weight isoform. The adjacent upstream wobble base was also changed from C to T so that the composite of these three base changes generated a PstI site that is diagnostic for the exchanged allele. Cells with the exchanged allele were selected for the loss of HPRT function using 5 mg/mL 6-thioguanine. There are no LoxP, Frt or marker gene sequences in this new allele. (J:94550)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 4 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Fgf2 Mutation:  14 strains or lines available
Notes
E14TG2a congenic line with one Fgf2 ko allele was used to produce this allele.
References
Original:  J:94550 Garmy-Susini B, et al., Role of fibroblast growth factor-2 isoforms in the effect of estradiol on endothelial cell migration and proliferation. Circ Res. 2004 May 28;94(10):1301-9
All:  9 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory