Tg(Ins2-Irs2)13Mfw
Transgene Detail

Summary

• Symbol: Tg(Ins2-Irs2)13Mfw
• Name: transgene insertion 13, Morris F White
• MGI ID: MGI:3510672
• Synonyms: rip13^Irs2
• Transgene: Tg(Ins2-Irs2)13Mfw Location: unknown
• Alliance: Tg(Ins2-Irs2)13Mfw page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-Irs2)13Mfw expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Irs2 (MGI:109334)

• Tg(Ins2-Irs2)13Mfw expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: This transgene consists of the Irs2 gene with a FLAG tag at the C terminus driven by the rat insulin II promoter. Two transgenes (lines 9 and 13) were analyzed based on expression level. This transgene expressed a high level of Irs2. Overexpression of the transgene was verified by Western blot analysis of islet extracts, immunostaining of pancreas and by an increase in the pancreatic insulin content. (J:94000)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:94000 Hennige AM, et al., Upregulation of insulin receptor substrate-2 in pancreatic beta cells prevents diabetes. J Clin Invest. 2003 Nov;112(10):1521-32
• All: 6 reference(s)