Tg(ABCG5/ABCG8)14-2Hobb
Transgene Detail

Summary

• Symbol: Tg(ABCG5/ABCG8)14-2Hobb
• Name: transgene insertion 14-2, Helen H Hobbs
• MGI ID: MGI:3057161
• Synonyms: ABCG5/ABCG8, G5G8^Tg, hG5G8Tg
• Transgene: Tg(ABCG5/ABCG8)14-2Hobb Location: unknown
• Alliance: Tg(ABCG5/ABCG8)14-2Hobb page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(ABCG5/ABCG8)14-2Hobb expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ABCG5 (64240)

  Abcg5

  (MGI:1351659)

  human

  ABCG8 (64241)

  Abcg8

  (MGI:1914720)

• Mutation details: The transgene contains a 90kb of sequence including the entire coding regions of the human ABCG5 and ABCG8 genes, approximately 30Kb of sequence flanking the 3' end of ABCG5, 6Kb of sequence flanking the 3' end of ABCG8 and sequence encoding exons 6 to 13 of a putative gene called CGI-60. Estimated transgene copy number is ~14 for this line. Northern blot analysis revealed the human transgene is expressed in the liver and small intestine. RT-PCR showed trace levels of transgene transcript in ovary tissue. (J:78963)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:78963 Yu L, et al., Overexpression of ABCG5 and ABCG8 promotes biliary cholesterol secretion and reduces fractional absorption of dietary cholesterol. J Clin Invest. 2002 Sep;110(5):671-80
• All: 5 reference(s)