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Tg(Hlxb9-GFP)1Tmj
Transgene Detail
Nomenclature
Symbol: Tg(Hlxb9-GFP)1Tmj
Name: transgene insertion 1, Thomas M Jessell
MGI ID: MGI:3056906
Synonyms: Gfp-HB9, Hb9-eGFP, Hb9::EGFP, Hb9-Gfp, Hb9:GFP-1B, Hlxb9:GFP
Transgene: Tg(Hlxb9-GFP)1Tmj  Location: unknown  Genetic Position: Chr15, Syntenic
Transgene
origin
Strain of Origin:  (C57BL/6 x CBA)F1
Transgene
description
Transgene Type:    Transgenic (Reporter)
Mutation:    Insertion
 
Mutation detailsThe transgenic construct contains a 9kb sequence of the 5' portion of the mouse Hlxb9 (Mnx1) gene, a 5' splice substrate, an enhanced Green Fluorescent Protein (EGFP) open reading frame, and a bovine growth hormome polyadenylation site sequence. Line 1 contains 10 to 11 tandem copies of the transgene. The expression pattern of this transgene is the same as that for the endogenous gene from which the promoter is derived. (J:88764, J:271391)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Notes
Homozygous transgenic mice on a genetic background that involves C57BL/6 and CBA are viable and fertile. They do not display any gross behavioral abnormalities, but are smaller in size than wildtype littermates. Homozygous pups born to homozygous females have a high mortality rate. Transgenic mice display distinct expression of GFP in dendrites, axons, and soma of spinal motor neurons, allowing identification, isolation and purification of spinal motor neurons by FACS. GFP expression mimics endogenous HLXB9 expression pattern. Fluorescence is detected in axons, dendrites and processes of spinal motor neurons at embryonic day 9.5 to postnatal day 10 aged mice.
References
Original:  J:88764 Wichterle H, et al., Directed differentiation of embryonic stem cells into motor neurons. Cell. 2002 Aug 9;110(3):385-97
All:  91 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/11/2019
MGI 6.14
The Jackson Laboratory