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Adgrg2tm1Bend
Targeted Allele Detail
Nomenclature
Symbol: Adgrg2tm1Bend
Name: adhesion G protein-coupled receptor G2; targeted mutation 1, Ben Davies
MGI ID: MGI:3056121
Synonyms: HE6-
Gene: Adgrg2  Location: ChrX:160390690-160498070 bp, + strand  Genetic Position: ChrX, 73.95 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:93019
Parent Cell Line:  E14TG2a (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA targeting vector was designed to replace exons 22 through 25 with a neomycin resistance gene, stop codons in all three reading frames, and an IRES-Beta-galactosidase cassette. The deleted sequence encoded the 7 transmembrane domain. Northern blot analysis of mutants indicated a lack of transcript for this domain. Two short, weakly expressed transcripts were present in mutants, one corresponding to exons 1-20 and the other corresponding to the truncated mRNA fused to Beta-gal. Ablation of wild-type protein was confirmed by Western blot of epididymal membrane samples, and lacZ activity was demonstrated by BLUO-GAL staining. (J:93019)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 7 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Adgrg2 Mutation:  3 strains or lines available
References
Original:  J:93019 Davies B, et al., Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation and male infertility. Mol Cell Biol. 2004 Oct;24(19):8642-8
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/16/2021
MGI 6.16
The Jackson Laboratory