Hdac5^tm1Eno
Targeted Allele Detail

Summary

• Symbol: Hdac5^tm1Eno
• Name: histone deacetylase 5; targeted mutation 1, Eric N Olson
• MGI ID: MGI:3056065
• Synonyms: Hdac5^-, Hdac^lacZ
• Gene: Hdac5 Location: Chr11:102085244-102120968 bp, - strand Genetic Position: Chr11, 65.82 cM
• Alliance: Hdac5^tm1Eno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93021
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to replace exons 3-7 with a lacZ-neo cassette. The deleted sequence encoded residues 32-298, including the region responsible for interaction with MEF2. RT-PCR and sequencing revealed that exon 2 was spliced to the 5' end of exon 3, which was spliced to the lacZ coding sequence. Transcripts of the wild-type were not found in hearts from mutants. Staining indicated that lacZ expression reflected that of the endogenous gene during pre- and postnatal development. (J:93021)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hdac5 Mutation: 56 strains or lines available

References

• Original: J:93021 Chang S, et al., Histone deacetylases 5 and 9 govern responsiveness of the heart to a subset of stress signals and play redundant roles in heart development. Mol Cell Biol. 2004 Oct;24(19):8467-76
• All: 28 reference(s)