Btrc^tm1Paga
Targeted Allele Detail

Summary

• Symbol: Btrc^tm1Paga
• Name: beta-transducin repeat containing protein; targeted mutation 1, Michele Pagano
• MGI ID: MGI:3055737
• Synonyms: BetaTrcp1^-
• Gene: Btrc Location: Chr19:45352173-45518452 bp, + strand Genetic Position: Chr19, 38.75 cM, cytoband D1
• Alliance: Btrc^tm1Paga page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:93010
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting event deleted a large segment of exon 5 that encodes amino acids 154-212 of the protein, including all but four amino acids comprising the F box and 22 downstream amino acids. The neomycin resistance gene is inserted in reverse orientation, and stop codons occur in all three reading frames within exon 5 following codon 153. The splice acceptor site of exon 5 is intact, and exons 6 and 7 are both out of frame with exon 4. No expression was detected in homozygous mutant mouse embryonic fibroblasts or other tissues examined by Northern blot analysis or by immunoprecipitation followed by immunoblot analysis. (J:93010)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Btrc Mutation: 47 strains or lines available

References

• Original: J:93010 Guardavaccaro D, et al., Control of meiotic and mitotic progression by the F box protein beta-Trcp1 in vivo. Dev Cell. 2003 Jun;4(6):799-812
• All: 4 reference(s)