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Rhotm1(EDNRB)Fji
Targeted Allele Detail
Nomenclature
Symbol: Rhotm1(EDNRB)Fji
Name: rhodopsin; targeted mutation 1, Yoshinori Fujiyoshi
MGI ID: MGI:3055163
Synonyms: hETBR-CT, Rhotm1(hETBR)Fji
Gene: Rho  Location: Chr6:115931748-115940036 bp, + strand  Genetic Position: Chr6, 53.72 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:98779
Parent Cell Line:  CJ7 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
Mutation:    Insertion
 
Mutation detailsExon 1 was replaced with cDNA encoding a human endothelial receptor type B fused to the C-terminal 10 amino acids of rhodopsin immediately before the stop codon and 0.9 kb of the 3' UTR sequence with three pA signals and a PGK-neo selection cassette. Northern blot of mutant demonstrated successful expression of the knock-in sequence under endogenous control sequences. Transcripts of three different lengths were detected, corresponding to location of the three pA signals. Quantitative RT-PCR detected levels of mutant transcripts were present at ~8.7% of wild-type levels. (J:98779)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rho Mutation:  13 strains or lines available
References
Original:  J:98779 Kodama T, et al., Expression and localization of an exogenous G protein-coupled receptor fused with the rhodopsin C-terminal sequence in the retinal rod cells of knockin mice. Exp Eye Res. 2005 Jun;80(6):859-69
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory