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Transgene Detail
Symbol: Tg(BCL2)25Wehi
Name: transgene insertion 25, Walter and Eliza Hall Institute of Medical Research
MGI ID: MGI:3052945
Synonyms: 25Wehi, Bcl2/Wehi25 Tg, Emu-2-25, Emu-bcl-2-25, H2k-Bcl2-tg, hBcl-2
Transgene: Tg(BCL2)25Wehi  Location: unknown  
Strain of Origin:  (C57BL/6JWehi x SJL/JWehi)F2
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(BCL2)25Wehi expresses 1 gene
Mutation detailsThe transgene construct consists of the human BCL2 cDNA in association with the Emu immunoglobulin heavy chain enhancer and SV40 promoter. (J:93111)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available

In transgenic mice on a C57BL/6 background, expression of the transgene is restricted to the T cell lineage (no B-cell expression).

Thymocytes, peripheral T-cells and activated T cells from these mice withstand prolonged culture in the absence of growth factors. Cells are resistant to killing by gamma-radiation, glucocorticoids, ionomycin, PMA, and sodium azide, but not complement, cytotoxic T cells or Fas ligand. Hemizygotes have a normal total T-cell count and thymic involution rate but show an enhanced response to immunization. This transgenic line displays no detectable autoimmunity. These mice serve as a robust source for the production of T-cell lines or hybridomas.

Original:  J:93111 Strasser A, et al., Abnormalities of the immune system induced by dysregulated bcl-2 expression in transgenic mice. Curr Top Microbiol Immunol. 1990;166:175-81
All:  56 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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