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Tg(Chx10-EGFP/cre,-ALPP)2Clc
Transgene Detail
Nomenclature
Symbol: Tg(Chx10-EGFP/cre,-ALPP)2Clc
Name: transgene insertion 2, Constance L Cepko
MGI ID: MGI:3052237
Synonyms: Chx10 BAC, Chx10 BAC line 2, Chx10-cre, Chx10::Cre, Tg(Chx10-EGFP/cre-ALPP)2Clc, Tg(Chx10-GFP/cre, ALPP)2Clc/J, Tg(Vsx2-EGFP/cre-ALPP)2Clc
Transgene: Tg(Chx10-EGFP/cre,-ALPP)2Clc  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6 and SJL
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
 
Mutation details
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Chx10     Summary of all recombinase alleles driven by Chx10.
 

Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
According to Rowan and Cepko (2004), the modified BAC DNA "was injected into male pronuclei of SJL/B6 fertilized eggs."

Two transgenic lines were generated. Line 1 exhibited highly mosaic expression of the EGFP reporter. Line 2 expressed EGFP more uniformly, but weakly; excision of the FRT-neo cassette increased EGFP expression in subsequent generations, so line 2 was used for further studies.

Hemizygous transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Alkaline phosphatase expression is mosaic, but specific to the retina, and is also detected in Muller glial cells. Green Fluorescent Protein (GFP) expression is detected in the outer neuroblastic layer of the retina at embryonic days 14.5, 17.5 and neonates. When crossed to a cre reporter strain, the resulting mice exhibit mosaicism in reporter gene expression.

References
Original:  J:91498 Rowan S, et al., Genetic analysis of the homeodomain transcription factor Chx10 in the retina using a novel multifunctional BAC transgenic mouse reporter. Dev Biol. 2004 Jul 15;271(2):388-402
All:  55 reference(s)

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last database update
08/12/2014
MGI 5.19
The Jackson Laboratory