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Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
Transgene Detail
Nomenclature
Symbol: Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck
Name: transgene insertion 2, Donald A Cohen
MGI ID: MGI:3051865
Synonyms: Mafia, MaFIA (Macrophage Fas-Induced Apoptosis), Tg(Csf1r-GFP, NGFR/FKBP12)2Bck
Transgene: Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6
Transgene
description
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck expresses 3 genes
 
Mutation detailsThe transgene's bicistronic transcript, expressed from the promoter of the macrophage-expressed colony stimulating factor 1 receptor gene, encodes enhanced green fluorescent protein (EGFP) and a conditionally apoptotic fusion protein. The latter comprises the extracellular and transmembrane domains of the human low affinity nerve growth factor receptor, two tandem copies of the human FK506 binding protein, and the intracellular domain of human TNFRSF6 - the Fas receptor protein - and is translated from an internal RNA expression signal (IRES) inserted at the translation start codon of the nerve growth factor receptor (NGFR) coding sequence. (J:89035)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Homozygous mutant mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

The transgene is expressed constitutively in macrophages and dendritic cells and permits conditional ablation of these cell populations in the mouse. Oligomerization of the fusion protein by binding of a bivalent FK506 analog causes them to undergo apoptosis via the Fas-mediated pathway.

In transgenic mice treated with the dimerizing reagent AP20187, EGFP fluorescing peritoneal and bone marrow macrophage numbers are depleted by more than 90%, and macrophage numbers in blood, spleen, lung and thymus by more than 70%. Seven days after cessation of treatment, the EGFP fluorescing macrophage and dendritic cell populations rebound.

References
Original:  J:89035 Burnett SH, et al., Conditional macrophage ablation in transgenic mice expressing a Fas-based suicide gene. J Leukoc Biol. 2004 Apr;75(4):612-23
All:  42 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/22/2020
MGI 6.16
The Jackson Laboratory