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Ghrhtm1Salv
Targeted Allele Detail
Summary
Symbol: Ghrhtm1Salv
Name: growth hormone releasing hormone; targeted mutation 1, Roberto Salvatori
MGI ID: MGI:3051561
Synonyms: GHRH-, GHRHKO
Gene: Ghrh  Location: Chr2:157171417-157189426 bp, - strand  Genetic Position: Chr2, 78.25 cM
Alliance: Ghrhtm1Salv page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:91828
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129/Sv
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsPart of intron 2, the last 3 amino acids of the N-terminal signal peptide and the first 14 amino acids of the mature protein, in exon 3, were replaced by a neomycin resistance gene. Southern blotting confirmed recombination in ES cells and in homozygous mice. RT-PCR indicated lack of transcript in mutant hypothalamus, testis and placenta. mRNA content in mutant pituitary was significantly less than that in wild-types and heterozygotes and Western blot confirmed this by showing a reduction in the intensity of protein in mutant pituitary and no protein in testes. (J:91828)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 4 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ghrh Mutation:  14 strains or lines available
References
Original:  J:91828 Alba M, et al., A mouse with targeted ablation of the growth hormone-releasing hormone gene: a new model of isolated growth hormone deficiency. Endocrinology. 2004 Sep;145(9):4134-43
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/12/2024
MGI 6.23
The Jackson Laboratory