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Targeted Allele Detail
Symbol: Atp2b4tm1Ges
Name: ATPase, Ca++ transporting, plasma membrane 4; targeted mutation 1, Gary E Shull
MGI ID: MGI:3051542
Synonyms: Pmca4-
Gene: Atp2b4  Location: Chr1:133699457-133801041 bp, - strand  Genetic Position: Chr1, 57.95 cM
Atp2b4tm1Ges/Atp2b4tm1Ges portal vein smooth muscle shows an increase in apoptosis

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:91844
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA region of exon 11 encoding amino acids 448-474, including the catalytic phosphorylation site (aspartic acid 465), was replaced with a neomycin resistance gene. Northern blot showed no reduction in mRNA levels in mutant mice. Exon-11-deficient mice express a mutant PMCA4 mRNA as a consequence of exon-10 splicing to exon-12, which preserves the reading frame and enables these mice to produce functionless mutant PMCA4a and PMCA4b proteins (devoid of a catalytic site). (J:91844, J:210683)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Atp2b4 Mutation:  31 strains or lines available
Original:  J:91844 Okunade GW, et al., Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4. J Biol Chem. 2004 Aug 6;279(32):33742-50
All:  9 reference(s)

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MGI 6.12
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