Cyb5r4^tm1Hfb
Targeted Allele Detail

Summary

• Symbol: Cyb5r4^tm1Hfb
• Name: cytochrome b5 reductase 4; targeted mutation 1, H Franklin Bunn
• MGI ID: MGI:3050973
• Synonyms: Ncb5or^-
• Gene: Cyb5r4 Location: Chr9:86904082-86959827 bp, + strand Genetic Position: Chr9, 46.69 cM
• Alliance: Cyb5r4^tm1Hfb page

Immunohistochemical and ultrastructural assessment of insulin- and glucagon-producing cells in Cyb5r4^tm1Hfb/Cyb5r4^tm1Hfb mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91486
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4, which contains sequence encoding the heme-binding domain, was replaced with a PGK-hygromycin gene. Mutant mice did not express full length mRNA or protein, as shown by Northern blot and Western blot, respectively. (J:91486)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Cyb5r4 Mutation: 41 strains or lines available

References

• Original: J:91486 Xie J, et al., Absence of a reductase, NCB5OR, causes insulin-deficient diabetes. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10750-5
• All: 6 reference(s)