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Targeted Allele Detail
Symbol: Nfat5tm1Snh
Name: nuclear factor of activated T cells 5; targeted mutation 1, Steffan N Ho
MGI ID: MGI:3050805
Synonyms: Nfat5delta
Gene: Nfat5  Location: Chr8:107293470-107379517 bp, + strand  Genetic Position: Chr8, 53.93 cM, cytoband D2
Germline Transmission:  Earliest citation of germline transmission: J:91482
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA floxed neo was inserted in intron 5 and a loxP site in intron 7. Transient Cre expression deleted the larger floxed region, including exons 6 and 7. These exons encode amino acids 254-380, which comprise the N-terminal portion of the rel DNA-binding domain (DBD). Germ line transmission was verified by PCR and Southern blot. RNA and protein expression in mutant MEFs showed that the allele encodes a truncated protein containing an internal deletion as expected. Immunoreactivity was eliminated as demonstrated by using antisera against the DBD. (J:91482)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nfat5 Mutation:  38 strains or lines available
Original:  J:91482 Go WY, et al., NFAT5/TonEBP mutant mice define osmotic stress as a critical feature of the lymphoid microenvironment. Proc Natl Acad Sci U S A. 2004 Jul 20;101(29):10673-8
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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