Cnot7^tm1Jpr
Targeted Allele Detail

Summary

• Symbol: Cnot7^tm1Jpr
• Name: CCR4-NOT transcription complex, subunit 7; targeted mutation 1, Jean-Pierre Rouault
• MGI ID: MGI:3050544
• Synonyms: mCAF1^-
• Gene: Cnot7 Location: Chr8:40945581-40968888 bp, - strand Genetic Position: Chr8, 23.89 cM
• Alliance: Cnot7^tm1Jpr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:91598
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Part of exon 2 as well as exons 3 and 4 were replaced with a lacZ-neo cassette. The deleted sequence included two-thirds of the protein, from the initiation codon to the alpha-helix-9 region and the main RNase catalytic site. Southern blot showed successful recombination and immunoblot analysis demonstrated lack of protein in mutants. (J:91598)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnot7 Mutation: 28 strains or lines available

References

• Original: J:91598 Berthet C, et al., CCR4-associated factor CAF1 is an essential factor for spermatogenesis. Mol Cell Biol. 2004 Jul;24(13):5808-20
• All: 1 reference(s)