Tg(Nes-Hspa1b-EGFP)25Okn
Transgene Detail

Summary

• Symbol: Tg(Nes-Hspa1b-EGFP)25Okn
• Name: transgene insertion 25, Hideyuki Okano
• MGI ID: MGI:3050084
• Synonyms: E/nestin:EGFP, E/nestin:EGFP25
• Transgene: Tg(Nes-Hspa1b-EGFP)25Okn Location: unknown
• Alliance: Tg(Nes-Hspa1b-EGFP)25Okn page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: Enhanced green fluorescent protein is expressed from the Hspa1b promoter under control of the second-intronic transcription enhancer of the rat nestin gene. Cells from embryonic brain that expressed high levels of EGFP included a subpopulation having characteristics of neural stem cells (NSCs) - capability for proliferation, self-renewal, and generation of both neural and glial cells - whereas few putative NSCs were observed among low- or non-expressing cells. In adult mice, EGFP expressing cells, which occur only in the periventricular region, include a population of slowly dividing, multipotential progenitor cells that likely represent adult stem cells. (J:91456)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Of six transgenic lines generated, only that designated #25 is described in the reference; it was noted that all lines had similar expression patterns.

References

• Original: J:91456 Kawaguchi A, et al., Nestin-EGFP transgenic mice: visualization of the self-renewal and multipotency of CNS stem cells. Mol Cell Neurosci. 2001 Feb;17(2):259-73
• All: 3 reference(s)