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Gabra6tm2(cre)Wwis
Targeted Allele Detail
Nomenclature
Symbol: Gabra6tm2(cre)Wwis
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6; targeted mutation 2, W Wisden
MGI ID: MGI:3047798
Synonyms: delta alpha6Cre
Gene: Gabra6  Location: Chr11:42306437-42321072 bp, - strand  Genetic Position: Chr11, 25.03 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:91624
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsExon 8 was disrupted by insertion, via homologous recombination, of a TAG3 IRES-nlsCre-neo cassette, which contains stop codons in all reading frames followed by an encephalomyelocarditis virus internal ribosome entry site (IRES), the coding sequence for Cre recombinase flanked by the nuclear localization sequence and polyadenylation signal from SV40, and a neomycin resistance gene driven by the HSV promoter. Cre was shown, both by immunohistochemistry and by X-gal staining of brains of adult F1 progeny from a cross to a beta-galactosidase expressing Cre reporter strain, to be expressed uniformly in nuclei of all cells of the granule cell layer and, additionally, in a few Purkinje cells and in some small cells in the molecular layer. (J:91624)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Gabra6     Summary of all recombinase alleles driven by Gabra6.
 

Phenotypes
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View phenotypes for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 6 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gabra6 Mutation:  7 strains or lines available
References
Original:  J:91624 Aller MI, et al., Cerebellar granule cell Cre recombinase expression. Genesis. 2003 Jun;36(2):97-103
All:  11 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory