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Targeted Allele Detail
Symbol: Prnp/Prndtm1Aag
Name: prion protein; targeted mutation 1, Adriano Aguzzi
MGI ID: MGI:3047635
Synonyms: Prno
Gene: Prnp  Location: Chr2:131909928-131938429 bp, + strand  Genetic Position: Chr2, 64.07 cM
Germline Transmission:  Earliest citation of germline transmission: J:89232
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Intergenic deletion
Mutation detailsUsing transalleleic targeted meiotic recombination, this allele was created in which both the Prnd and Prnp loci are disrupted. The recombined allele lacks ~16 kb encompassing both genes. To generate this allele, Prnptm2.1Cwe mice, which have a loxP site in place of the coding sequence, were crossed with Prndtm1Aag mice, which have a floxed neo in place of the coding exon. Northern blot analysis indicated absence of Prnp and Prnd transcript in brain, spleen and testes of mutants. (J:89232)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prnp Mutation:  87 strains or lines available
Original:  J:89232 Genoud N, et al., Disruption of Doppel prevents neurodegeneration in mice with extensive Prnp deletions. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4198-203
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory