Prnp/Prnd^tm1Aag
Targeted Allele Detail

Summary

• Symbol: Prnp/Prnd^tm1Aag
• Name: prion protein; targeted mutation 1, Adriano Aguzzi
• MGI ID: MGI:3047635
• Synonyms: Prn^o
• Gene: Prnp Location: Chr2:131751848-131780349 bp, + strand Genetic Position: Chr2, 64.07 cM
• Alliance: Prnp/Prnd^tm1Aag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89232
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intergenic deletion
• Mutation details: Using transalleleic targeted meiotic recombination, this allele was created in which both the Prnd and Prnp loci are disrupted. The recombined allele lacks ~16 kb encompassing both genes. To generate this allele, Prnp^tm2.1Cwe mice, which have a loxP site in place of the coding sequence, were crossed with Prnd^tm1Aag mice, which have a floxed neo in place of the coding exon. Northern blot analysis indicated absence of Prnp and Prnd transcript in brain, spleen and testes of mutants. (J:89232)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prnp Mutation: 150 strains or lines available

References

• Original: J:89232 Genoud N, et al., Disruption of Doppel prevents neurodegeneration in mice with extensive Prnp deletions. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4198-203
• All: 3 reference(s)