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Targeted Allele Detail
Symbol: Rhotm2(RHO/GFP)Jhw
Name: rhodopsin; targeted mutation 2, John H Wilson
MGI ID: MGI:3046186
Synonyms: hrhoG, RGFP, Rho-eGFP
Gene: Rho  Location: Chr6:115908709-115916997 bp, + strand  Genetic Position: Chr6, 53.72 cM
Germline Transmission:  Earliest citation of germline transmission: J:91038
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Rhotm2(RHO/GFP)Jhw expresses 1 gene
Mutation detailsThe endogenous gene was first replaced with an HPRT minigene and then the HPRT minigene was replaced with the human RHO gene fused to GFP via homologous recombination. Western blot analysis indicated that expression of the human gene was about 80% that of the endogenous gene. GFP expression is seen in the eyes. (J:91038)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rho Mutation:  37 strains or lines available
Original:  J:91038 Chan F, et al., Knock-in human rhodopsin-GFP fusions as mouse models for human disease and targets for gene therapy. Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):9109-14
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.21
The Jackson Laboratory