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Tg(TcraTcrb)425Cbn
Transgene Detail
Nomenclature
Symbol: Tg(TcraTcrb)425Cbn
Name: transgene insertion 425, Frank Carbone
MGI ID: MGI:3046083
Synonyms: OT2, OT-2, OTII, OT-II, OT-II.2, OT-II alphabeta TCR, OTII TCR, TCR-OT-II.2a, TCRTg, TgN(OT-II.2a), TgN(TcrOT-II), Tg(TcraTcrb)425-2Cbn
Transgene: Tg(TcraTcrb)425Cbn  Location: unknown  
Transgene
origin
Strain of Origin:  C57BL/6 x B6.C-H2bm1
Transgene
description
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
 
Tg(TcraTcrb)425Cbn expresses 2 genes
 
Mutation detailsThe transgene was generated by co-injection of two constructs created by separate cloning into a T-cell receptor expression vector the PCR-amplified cDNAs encoding mouse alpha-chain and beta-chain T-cell receptor subunits expressed by a CD4+ T-cell hybridoma designated Clone 1.1. The reconstituted receptor pairs with the CD4 coreceptor and is specific for chicken ovalbumin residues 323-339 in the context of H2-Ab1 (I-Ab). (J:87876)
Phenotypes
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Notes
Transgenic mice express the mouse alpha-chain and beta-chain T-cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous transgenic mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T-cell ratio, and lymph node T-cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. Phenotypes for the two sublines (OT-II.2)425-2 and 426-6 are described as identical. (J:87876)

References
Original:  J:87876 Barnden MJ, et al., Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol. 1998 Feb;76(1):34-40
All:  868 reference(s)

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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory