Slc14a2^tm1Raf
Targeted Allele Detail

Summary

• Symbol: Slc14a2^tm1Raf
• Name: solute carrier family 14 (urea transporter), member 2; targeted mutation 1, Robert A Fenton
• MGI ID: MGI:3043851
• Synonyms: UT-A1/3 -
• Gene: Slc14a2 Location: Chr18:78189363-78640157 bp, - strand Genetic Position: Chr18, 52.53 cM
• Alliance: Slc14a2^tm1Raf page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:89834
• Parent Cell Line: CMTI-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 10 was replaced with a pgk-neo cassette via homologous recombination. This selectively deleted two of the isoforms (UT-A1 and UT-A3). Immunoblot analysis confirmed the absence of the UT-A1 and UT-A3 proteins and the presence of the UT-A2 protein. A fourth testis specific isoform, UT-A5, also contains exon 10 and is predicted to be deleted. (J:89834)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc14a2 Mutation: 53 strains or lines available

References

• Original: J:89834 Fenton RA, et al., Urinary concentrating defect in mice with selective deletion of phloretin-sensitive urea transporters in the renal collecting duct. Proc Natl Acad Sci U S A. 2004 May 11;101(19):7469-74
• All: 9 reference(s)