Junb^tm3Wag
Targeted Allele Detail

Summary

• Symbol: Junb^tm3Wag
• Name: jun B proto-oncogene; targeted mutation 3, Erwin F Wagner
• MGI ID: MGI:3036209
• Synonyms: junB^delta, junB^f, JunBtmAngl
• Gene: Junb Location: Chr8:85703538-85705377 bp, - strand Genetic Position: Chr8, 41.41 cM, cytoband C2-D1
• Alliance: Junb^tm3Wag page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:88126
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed and frt-flanked selection cassette containing both a neomycin resistance gene and a thymidine kinase gene was inserted in the 5' untranslated region. An additional loxP site was inserted 161 bp downstream of the translation stop codon. The neomycin and thymidine kinase genes were subsequently deleted from properly targeted ES cells by transient transfection with a flp recombinase expressing vector leaving the coding sequence flanked by loxP sites. Northern blot and Western blot analysis confirmed the absence of both transcripts and protein product. (J:88126)

Disease models

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Junb Mutation: 19 strains or lines available

References

• Original: J:88126 Kenner L, et al., Mice lacking JunB are osteopenic due to cell-autonomous osteoblast and osteoclast defects. J Cell Biol. 2004 Feb 16;164(4):613-23
• All: 43 reference(s)