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Efnb3tm1.1Henk
Targeted Allele Detail
Summary
Symbol: Efnb3tm1.1Henk
Name: ephrin B3; targeted mutation 1.1, Mark Henkemeyer
MGI ID: MGI:3026702
Synonyms: ephrin3lacZ, ephrin-B3lacZ
Gene: Efnb3  Location: Chr11:69444918-69451031 bp, - strand  Genetic Position: Chr11, 42.8 cM
Alliance: Efnb3tm1.1Henk page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:87497
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe frt-flanked neo cassette was excised from Efnb2tm1Henk via FLP-mediated recombination, leaving the loxP flanked lacZ cassette in exon 5. The lacZ cassette disrupted codons 264 through 340 which make up the majority of the cytoplasmic domain. Biochemical analysis indicated that the expected EFNB2-beta-gal protein, in which the endogenous cytoplasmic domain is replaced with beta-gal, was produced. This mutation leaves forward signaling intact and ablates reverse signaling. The expression pattern of lacZ was observed to be to similar to that of the endogenous gene. (J:87497)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Efnb3 Mutation:  11 strains or lines available
References
Original:  J:87497 Yokoyama N, et al., Forward signaling mediated by ephrin-B3 prevents contralateral corticospinal axons from recrossing the spinal cord midline. Neuron. 2001 Jan;29(1):85-97
All:  17 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory