Nid1^tm1Dong
Targeted Allele Detail

Summary

• Symbol: Nid1^tm1Dong
• Name: nidogen 1; targeted mutation 1, Lijin Dong
• MGI ID: MGI:2682959
• Synonyms: TV2
• Gene: Nid1 Location: Chr13:13612252-13686849 bp, + strand Genetic Position: Chr13, 5.26 cM
• Alliance: Nid1^tm1Dong page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:80843
• Parent Cell Line: GK129 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment encompassing a portion of the promoter and exon 1 of the functional locus was replaced with a hygromycin selection cassette inserted by homologous recombination. Transcript was undetected by Northern blot and RT-PCR analyses of RNA obtained from homozygous mutant skeletal muscle. Western blot analysis of homozygous mutant ES cells indicated an absence of protein both in the absence and presence of retinoic acid. (J:80843)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nid1 Mutation: 63 strains or lines available

References

• Original: J:80843 Dong L, et al., Neurologic defects and selective disruption of basement membranes in mice lacking entactin-1/nidogen-1. Lab Invest. 2002 Dec;82(12):1617-30
• All: 2 reference(s)