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Transgene Detail
Symbol: Tg(PLFSADA)2465Rkmb
Name: transgene insertion 2465, Michael R Blackburn
MGI ID: MGI:2681791
Synonyms: Tg(Ada)2448Mw
Transgene: Tg(PLFSADA)2465Rkmb  Location: unknown  
Strain of Origin:  FVB/N
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(PLFSADA)2465Rkmb expresses 1 gene
Mutation detailsA construct was made that included a 2.8 kb genomic fragment containing the endogenous polyadenylation sequence, intron 11, and ~2kb of 3'-untranslated region and 3' flanking region of the gene. The endogenous promoter was replaced with a promoter containing a 36-bp deletion in the 5'-untranslated region. This resulted in a construct that contained the gene's regulatory elements, allowing expression in the placenta prenatally and the forestomach postnatally, while the 36-bp deletion in the promoter allowed distinction of this transcript from that of native transcript. RNase protection showed transgene transcript in placentas, but not fetuses, of transgenic animals. Enzymatic activity of the protein product was increased in the placenta, but not in the fetus, of transgenic mice compared to nontransgenic animals. (J:29350)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:29350 Blackburn MR, et al., Tissue-specific rescue suggests that placental adenosine deaminase is important for fetal development in mice. J Biol Chem. 1995 Oct 13;270(41):23891-4
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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