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Targeted Allele Detail
Symbol: Mapkapk5tm1Mgl
Name: MAP kinase-activated protein kinase 5; targeted mutation 1, Matthias Gaestel
MGI ID: MGI:2680287
Synonyms: deltaex6, MK5-
Gene: Mapkapk5  Location: Chr5:121525051-121545892 bp, - strand  Genetic Position: Chr5, 61.84 cM, cytoband F
Germline Transmission:  Earliest citation of germline transmission: J:86268
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 6 was replaced with a neo cassette. The targeted exon encoded amino acids 131 through 161 which make up portions of the kinase catalytic core subdomains VIa and VIb. Northern blot and RT-PCR analyses identified mutant transcript approximately 100 bp shorter than normal transcript. Sequence analysis showed the mutant transcript to contain neither exon 6 nor the neo cassette. Low levels of truncated protein were detected by Western blot analysis, but this protein was reported to lack catalytic activity. (J:86268)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mapkapk5 Mutation:  22 strains or lines available
Original:  J:86268 Shi Y, et al., Elimination of protein kinase MK5/PRAK activity by targeted homologous recombination. Mol Cell Biol. 2003 Nov;23(21):7732-41
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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