Ep300^tm1Reck
Targeted Allele Detail

Summary

• Symbol: Ep300^tm1Reck
• Name: E1A binding protein p300; targeted mutation 1, Richard Eckner
• MGI ID: MGI:2679374
• Synonyms: p300^AS
• Gene: Ep300 Location: Chr15:81470329-81536278 bp, + strand Genetic Position: Chr15, 38.16 cM
• Alliance: Ep300^tm1Reck page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:85950
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Dominant negative, Null/knockout)
• Mutation: Nucleotide substitutions
• Mutation details: Acetyltransferase (AT) activity was ablated via the incorporation of engineered point mutations at the endogenous locus. The mutations substitute alanine and serine for tryptophan and tyrosine (WY(1466-1477)AS). A single loxP site remained in the intron following the missense mutations after the excision of floxed neo cassette used for selection. Western blot analysis indicated mutant protein was expressed at wild-type levels. (J:85950)
• Inheritance: Dominant

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ep300 Mutation: 97 strains or lines available

References

• Original: J:85950 Roth JF, et al., Differential role of p300 and CBP acetyltransferase during myogenesis: p300 acts upstream of MyoD and Myf5. EMBO J. 2003 Oct 1;22(19):5186-96
• All: 2 reference(s)