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Opn1mwtm1(OPN1LW)Nat
Targeted Allele Detail
Nomenclature
Symbol: Opn1mwtm1(OPN1LW)Nat
Name: opsin 1 (cone pigments), medium-wave-sensitive (color blindness, deutan); targeted mutation 1, Jeremy Nathans
MGI ID: MGI:2678763
Synonyms: Opn1mwR, Opn1mwred, R
Gene: Opn1mw  Location: ChrX:73171072-73194362 bp, + strand  Genetic Position: ChrX, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:85849
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Mutation
description
Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsHuman cDNA encoding OPN1LW was inserted at the endogenous mouse Opn1mw locus, replacing a 3' portion of exon 2 and all of exons 3, 4, and 5. The allele putatively produces a hybrid protein consisting of 92 N-terminal residues encoded by mouse exon 1 and the 5' portion of exon 2, and 266 C-terminal residues encoded by the human cDNA. Nearly the entire chromophore binding pocket, as well as the residues that account for the difference in absorbance spectra between human and mouse, is derived from the human sequence. Immunostaining indicated expression of the hybrid protein and electroretinogram indicated that the spectral sensitivity of cones containing the targeted allele was similar to that of human red cones. (J:85849)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Opn1mw Mutation:  4 strains or lines available
References
Original:  J:85849 Smallwood PM, et al., Genetically engineered mice with an additional class of cone photoreceptors: implications for the evolution of color vision. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11706-11
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory