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Targeted Allele Detail
Symbol: Thrbtm3Few
Name: thyroid hormone receptor beta; targeted mutation 3, Frederic E Wondisford
MGI ID: MGI:2676923
Synonyms: GS125 KI, TR-BetaGS
Gene: Thrb  Location: Chr14:17660960-18038086 bp, + strand  Genetic Position: Chr14, 7.08 cM, cytoband A3
Germline Transmission:  Earliest citation of germline transmission: J:85158
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted
Mutation:    Other
Mutation detailsMissense mutations were introduced at codons 125 and 126 (exon 3), resulting in Glu to Gly and Gly to Ser substitutions. The substitutions were within the P-box of the first zinc finger and were shown, in vitro, to abolish DNA-binding while retaining the ability to interact with T3 and cofactors. The mutations were verified by sequencing RT-PCR products. The self-excision of a neo cassette from intron 3 was confirmed, leaving a single loxP site. Western blot analysis showed endogenous levels of protein in homozygous mutant mice. (J:85158)
View phenotypes for all genotypes (concatenated display).
Disease models
In Mice Carrying this Mutation: 4 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Thrb Mutation:  15 strains or lines available
Original:  J:85158 Shibusawa N, et al., Thyroid hormone action in the absence of thyroid hormone receptor DNA-binding in vivo. J Clin Invest. 2003 Aug;112(4):588-97
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.04
The Jackson Laboratory