Thra^tm3Jas
Targeted Allele Detail

Summary

• Symbol: Thra^tm3Jas
• Name: thyroid hormone receptor alpha; targeted mutation 1, Jacques Samarut
• MGI ID: MGI:2670368
• Synonyms: TRalpha⁷
• Gene: Thra Location: Chr11:98631539-98659832 bp, + strand Genetic Position: Chr11, 62.58 cM, cytoband D-E
• Alliance: Thra^tm3Jas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84537
• Parent Cell Line: ENS (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Disruption caused by insertion of vector
• Mutation details: A loxP flanked cassette containing neo and tk genes replaced a 257 bp fragment of intron 7, eliminating a portion of the internal promoter that drives expression of two short isoforms, TRdeltaalpha1 and TRdeltaalpha2. The floxed selection genes were excised prior to germline transmission. Whereas wild-type levels of transcript encoding the two longer isoforms, TRalpha1 and TRalpha2, were detected in homozygous mutant mice, the levels of transcript encoding the shorter isoforms were reduced. (J:84537)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Thra Mutation: 39 strains or lines available

References

• Original: J:84537 Plateroti M, et al., Functional interference between thyroid hormone receptor alpha (TRalpha) and natural truncated TRDeltaalpha isoforms in the control of intestine development. Mol Cell Biol. 2001 Jul;21(14):4761-72
• All: 1 reference(s)