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Targeted Allele Detail
Symbol: Cx3cr1tm1Litt
Name: chemokine (C-X3-C motif) receptor 1; targeted mutation 1, Dan R Littman
MGI ID: MGI:2670351
Synonyms: CX3CR1-, CX3CR1EGFP, CX3CR1-EGFP, Cx3cr1gfp, CX3CR1GFP, CX3CR1-GFP, CX3CR-GFP
Gene: Cx3cr1  Location: Chr9:120048683-120068296 bp, - strand  Genetic Position: Chr9, 71.37 cM
Retinal lesion in Ccl2tm1Rol/Ccl2tm1Rol Cx3cr1tm1Litt/Cx3cr1tm1Litt mice

Show the 4 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:84544
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe endogenous locus was disrupted by the insertion of sequence encoding green fluourescent protein (GFP), replacing the first 390 bp of the coding exon (exon 2). The deleted region encoded an amino-terminal portion of the protein that is crucial for interaction with endogenous ligand, Cx3cl1. A floxed neo gene included in the targeting vector for selection was excised prior to germline transmission, leaving a single loxP site downstream of the GFP sequence. RT-PCR and flow cytometry indicated an absence of endogenous protein and the presence GFP expression in homozygous mutant mice. (J:84544)
View phenotypes for all genotypes (concatenated display).
Disease models
In Mice Carrying this Mutation: 2 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Cx3cr1 Mutation:  12 strains or lines available
Phenotypic Similarity to Human Syndrome: Macular Degeneration, Geographic Atrophy, Dry Type (J:200877)

Original:  J:84544 Jung S, et al., Analysis of fractalkine receptor CX(3)CR1 function by targeted deletion and green fluorescent protein reporter gene insertion. Mol Cell Biol. 2000 Jun;20(11):4106-14
All:  271 reference(s)

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MGI 6.01
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