Tgfbr3^tm1Stv
Targeted Allele Detail

Summary

• Symbol: Tgfbr3^tm1Stv
• Name: transforming growth factor, beta receptor III; targeted mutation 1, Kaye Stenvers
• MGI ID: MGI:2664514
• Synonyms: betaglycan^-, TBRIII^-
• Gene: Tgfbr3 Location: Chr5:107254436-107437495 bp, - strand Genetic Position: Chr5, 51.9 cM
• Alliance: Tgfbr3^tm1Stv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:83727
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-Neo expression cassette was inserted in the "sense" orientation into exon 2. Homologous recombination was verified by Southern blot analysis. Two weak bands representing aberrent transcripts were seen in northern blots of mRNA from embryos homozygous from the targeted gene. RT-PCR analysis confirmed that none of these transcripts represented wild-type mRNA and affinity labelling of embryo fibroblasts with TGFbetaI indicated that there was no binding to TGFbetaRIII. (J:83727)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tgfbr3 Mutation: 50 strains or lines available

References

• Original: J:83727 Stenvers KL, et al., Heart and liver defects and reduced transforming growth factor beta2 sensitivity in transforming growth factor beta type III receptor-deficient embryos. Mol Cell Biol. 2003 Jun;23(12):4371-85
• All: 5 reference(s)