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Prkcqtm1.1Bai
Targeted Allele Detail
Nomenclature
Symbol: Prkcqtm1.1Bai
Name: protein kinase C, theta; targeted mutation 1.1, Gottfried Baier
MGI ID: MGI:2664377
Synonyms: PKCTheta-, PKCThetaDelta
Gene: Prkcq  Location: Chr2:11172108-11301222 bp, + strand  Genetic Position: Chr2, 8.42 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:83733
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe floxed region encompassing about 10 kb of sequence, including a neo cassette and exons 3 and 4, was excised via cre mediated recombination in the germline. The deletion eliminated sequence encoding amino acids 10 through 87 and resulted in a frameshift mutation after codon 9. Western blot analysis indicated an absence of protein in thymocytes and CD3+ T cells. Sequence and splice sites corresponding to the testis-expressed isoform were left intact. (J:83733)
Phenotypes
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Prkcq Mutation:  28 strains or lines available
References
Original:  J:83733 Pfeifhofer C, et al., Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells. J Exp Med. 2003 Jun 2;197(11):1525-35
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory