Brca2^tm1Hst
Targeted Allele Detail

Summary

• Symbol: Brca2^tm1Hst
• Name: breast cancer 2, early onset; targeted mutation 1, Paul Hasty
• MGI ID: MGI:2653913
• Synonyms: brca2^lex1
• Gene: Brca2 Location: Chr5:150446095-150493794 bp, + strand Genetic Position: Chr5, 89.52 cM
• Alliance: Brca2^tm1Hst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82434
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A hypomorphic allele was generated by the replacement of exon 27 (coding nt 9418 through 9984) with an HPRT minigene. RT-PCR analysis identified a transcript lacking the targeted sequence encoding the RAD51-interacting domain. Truncated protein is translated and the ability of the protein to bind RAD51 is retained, putatively via the BRC motifs encoded by exon 11. The mutant protein contains more than 94% of the wild-type amino acid sequence. (J:49518, J:82434)

Tumor Data

List all tumor models in MMHCdb carrying Brca2^tm1Hst

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brca2 Mutation: 132 strains or lines available

References

• Original: J:82434 Donoho G, et al., Deletion of Brca2 exon 27 causes hypersensitivity to DNA crosslinks, chromosomal instability, and reduced life span in mice. Genes Chromosomes Cancer. 2003 Apr;36(4):317-31
• All: 3 reference(s)