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Targeted Allele Detail
Symbol: Brca2tm1Hst
Name: breast cancer 2, early onset; targeted mutation 1, Paul Hasty
MGI ID: MGI:2653913
Synonyms: brca2lex1
Gene: Brca2  Location: Chr5:150522630-150570329 bp, + strand  Genetic Position: Chr5, 89.52 cM
Germline Transmission:  Earliest citation of germline transmission: J:82434
Parent Cell Line:  AB2.2 (ES Cell)
Strain of Origin:  129S7/SvEvBrd-Hprtb-m2
Allele Type:    Targeted (Hypomorph)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA hypomorphic allele was generated by the replacement of exon 27 (coding nt 9418 through 9984) with an HPRT minigene. RT-PCR analysis identified a transcript lacking the targeted sequence encoding the RAD51-interacting domain. Truncated protein is translated and the ability of the protein to bind RAD51 is retained, putatively via the BRC motifs encoded by exon 11. The mutant protein contains more than 94% of the wild-type amino acid sequence. (J:49518, J:82434)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Brca2 Mutation:  62 strains or lines available
Original:  J:82434 Donoho G, et al., Deletion of Brca2 exon 27 causes hypersensitivity to DNA crosslinks, chromosomal instability, and reduced life span in mice. Genes Chromosomes Cancer. 2003 Apr;36(4):317-31
All:  3 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.06
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