Tg(Myh11-cre,-EGFP)2Mik
Transgene Detail

Summary

• Symbol: Tg(Myh11-cre,-EGFP)2Mik
• Name: transgene insertion 2, Michael I Kotlikoff
• MGI ID: MGI:2653286
• Synonyms: MH, smMHC-Cre, smMHC^Cre/eGFP, smMHC/Cre/eGFP, smMHC-Cre-GFP^tg, smMHC-Cre-IRES-eGFP, Tg(Myh11-cre/EGFP)1Mik
• Transgene: Tg(Myh11-cre,-EGFP)2Mik Location: unknown
• Alliance: Tg(Myh11-cre,-EGFP)2Mik page

Shorter small intestines in mgi:5563563/mgi:5563563 mgi:2653286/0 and mgi:5563563/mgi:5563564 mgi:2653286/0 mice

Show the 2 phenotype image(s) involving this allele.

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Recombinase, Reporter)
• Mutation: Insertion
• Tg(Myh11-cre,-EGFP)2Mik expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Myh11 (MGI:102643)

• Mutation details: The transgene consists of a mouse Myh11 promoter, a cre recombinase gene, an internal ribosome entry site (IRES) and an enhanced GFP gene. 16 kb of mouse Myh11 promoter sequences was used to drive the bicistronic transgene expression in smooth muscle cells. The promoter has also been reported to be active in female germ cells. A low frequency of deletion events are also observed by inheritance from the male germline. (J:81358, J:138838)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Myh11 (mouse) Summary of all recombinase alleles driven by Myh11. Comparative matrix view of recombinase activities Alliance mouse Myh11 gene page

Expression

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Two transgenic founders, SMCG2 and SMCG3, were created. Transgenic founders were bred to C57BL/6 mice.

References

• Original: J:81358 Xin HB, et al., Smooth muscle expression of Cre recombinase and eGFP in transgenic mice. Physiol Genomics. 2002 Sep 3;10(3):211-5
• All: 76 reference(s)