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Targeted Allele Detail
Symbol: Krt14tm2Der
Name: keratin 14; targeted mutation 2, Dennis R Roop
MGI ID: MGI:2653130
Synonyms: mtK14loxP
Gene: Krt14  Location: Chr11:100203162-100207510 bp, - strand  Genetic Position: Chr11, 63.43 cM, cytoband D
Blistering on the left front leg and abdomen of Krt14tm2Der/Krt14+ pups

Show the 2 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:67320
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted (Inducible, Inserted expressed sequence)
Mutation:    Insertion
Mutation detailsA C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. Transient expression of cre recombinase in correctly targeted ES cells deleted the floxed PGK-neo cassette leaving a single loxP site in its place. The targeted allele was confirmed by Southern blot and sequence analysis. (J:67320)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Krt14 Mutation:  5 strains or lines available
Original:  J:67320 Cao T, et al., An Inducible Mouse Model for Epidermolysis Bullosa Simplex. Implications for gene therapy. J Cell Biol. 2001 Feb 5;152(3):651-6
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.14
The Jackson Laboratory