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Oprm1tm2.1Loh
Targeted Allele Detail
Nomenclature
Symbol: Oprm1tm2.1Loh
Name: opioid receptor, mu 1; targeted mutation 2.1, Horace H Loh
MGI ID: MGI:2651420
Synonyms: S196A
Gene: Oprm1  Location: Chr10:6758593-7038209 bp, + strand  Genetic Position: Chr10, 1.85 cM, cytoband A2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:81978
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted
Mutation:    Nucleotide substitutions
 
Mutation detailsThe intronic neo cassette was excised from Oprmtm2Loh via in vivo cre mediated recombination, leaving the engineered serine to alanine missense mutation in exon 2 at codon 196 (S196A) intact. S196A affects the fourth transmembrane domain. A radioactive ligand receptor-binding assay showed reduced expression of the mutant protein in homozygous mutant mice, approximately 85% that of the endogenous protein in wild-type mice. The reduced protein expression correlated with reduced levels of transcript. (J:81978)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Oprm1 Mutation:  35 strains or lines available
Notes
ES cell line = SP1.
References
Original:  J:81978 Yang W, et al., In vivo activation of a mutant mu-opioid receptor by antagonist: future direction for opiate pain treatment paradigm that lacks undesirable side effects. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2117-21
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/12/2019
MGI 6.14
The Jackson Laboratory