Oprm1^tm2.1Loh
Targeted Allele Detail

Summary

• Symbol: Oprm1^tm2.1Loh
• Name: opioid receptor, mu 1; targeted mutation 2.1, Horace H Loh
• MGI ID: MGI:2651420
• Synonyms: S196A
• Gene: Oprm1 Location: Chr10:6708593-6988209 bp, + strand Genetic Position: Chr10, 1.85 cM, cytoband A2
• Alliance: Oprm1^tm2.1Loh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:81978
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: The intronic neo cassette was excised from Oprm^tm2Loh via in vivo cre mediated recombination, leaving the engineered serine to alanine missense mutation in exon 2 at codon 196 (S196A) intact. S196A affects the fourth transmembrane domain. A radioactive ligand receptor-binding assay showed reduced expression of the mutant protein in homozygous mutant mice, approximately 85% that of the endogenous protein in wild-type mice. The reduced protein expression correlated with reduced levels of transcript. (J:81978)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Oprm1 Mutation: 53 strains or lines available

Notes

ES cell line = SP1.

References

• Original: J:81978 Yang W, et al., In vivo activation of a mutant mu-opioid receptor by antagonist: future direction for opiate pain treatment paradigm that lacks undesirable side effects. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2117-21
• All: 2 reference(s)