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Hcn2tm1Ldw
Targeted Allele Detail
Nomenclature
Symbol: Hcn2tm1Ldw
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2; targeted mutation 1, Andreas Ludwig
MGI ID: MGI:2449917
Synonyms: HCN2-
Gene: Hcn2  Location: Chr10:79716634-79736108 bp, + strand  Genetic Position: Chr10, 39.72 cM, cytoband C1
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:81398
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA floxed neo cassette and a single loxP site flanking exons 2 and 3 were introduced via homologous recombination. The floxed neo cassette and exons 2 and 3 were removed by transient expression of cre recombinase in correctly targeted cells, resulting in deletion of sequences encoding 5 of the 6 transmembrane segments of the Hcn2 channel as well as part of the pore. Absence of gene expression was confirmed by Northern and Western blot analysis of brain extracts from homozygous mutant animals. (J:81398)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Hcn2 Mutation:  2 strains or lines available
References
Original:  J:81398 Ludwig A, et al., Absence epilepsy and sinus dysrhythmia in mice lacking the pacemaker channel HCN2. EMBO J. 2003 Jan 15;22(2):216-24
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/05/2019
MGI 6.14
The Jackson Laboratory