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Targeted Allele Detail
Symbol: Hspg2tm1Soin
Name: perlecan (heparan sulfate proteoglycan 2); targeted mutation 1, Raija Soininen
MGI ID: MGI:2449915
Synonyms: Hspg2delta3
Gene: Hspg2  Location: Chr4:137468769-137570630 bp, + strand  Genetic Position: Chr4, 69.93 cM
Germline Transmission:  Earliest citation of germline transmission: J:81399
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsExon 3 and flanking regions were deleted and replaced with a PGK-neo cassette via homologous recombination. The targeted mutation removes the heparan sulfate attachment while the core protein remains intact. RT-PC analysis of RNA from homozygous mutant embryos confirmed the exon 3 deletion. Fibroblasts derived from homozygous mutant cell culture were treated with heparitinase and chondroitinase, run on an SDS-PAGE gel, and immunoblotted to confirm the mutant protein retained the core protein and lacked heparan sulfate side chains. (J:81399)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Hspg2 Mutation:  212 strains or lines available
Original:  J:81399 Rossi M, et al., Heparan sulfate chains of perlecan are indispensable in the lens capsule but not in the kidney. EMBO J. 2003 Jan 15;22(2):236-45
All:  7 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.06
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