Sbf1^tm1Mlc
Targeted Allele Detail

Summary

• Symbol: Sbf1^tm1Mlc
• Name: SET binding factor 1; targeted mutation 1, Michael L Cleary
• MGI ID: MGI:2449174
• Synonyms: Sbf1^-
• Gene: Sbf1 Location: Chr15:89172439-89199514 bp, - strand Genetic Position: Chr15, 44.79 cM, cytoband F1
• Alliance: Sbf1^tm1Mlc page

Failure of spermatogenesis in Sbf1^tm1Mlc/Sbf1^tm1Mlc mice

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:76406
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exons 21-28 with a PGK-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Western blot analysis of brain extracts. Immunohistochemical analysis of seminferous tubules from homozygous mutant mice demonstrated absence of protein product in spermatogonia, pachytene spermatocytes, and Sertoli cells. (J:76406)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sbf1 Mutation: 43 strains or lines available

References

• Original: J:76406 Firestein R, et al., Male infertility, impaired spermatogenesis, and azoospermia in mice deficient for the pseudophosphatase Sbf1. J Clin Invest. 2002 May;109(9):1165-72
• All: 1 reference(s)