Tg(ACTFLPe)9205Dym
Transgene Detail

Summary

• Symbol: Tg(ACTFLPe)9205Dym
• Name: transgene insertion 9205, Susan Dymecki
• MGI ID: MGI:2448985
• Synonyms: ACTB-Flpe, ACTB:FLPe, ACTB::FLPe, ACTFlpe, ACT-FLPe, actin-FLPe, beta-actinFLPe, CAGG-Flpr, CAGGS-flp, Flp, Flp deleter, FLPe, hACTB-FLPe, hACTB::FLPe, Tg(ACTB-flpe)9205Dym, Tg(ACTB-FlpE)9205Dym, Tg (ACTFLEe)9205Dym, TgaFlpe, TgpCAG-flpe
• Transgene: Tg(ACTFLPe)9205Dym Location: unknown
• Alliance: Tg(ACTFLPe)9205Dym page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)F2

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(ACTFLPe)9205Dym expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  human

  ACTB (60)

  Actb	(MGI:87904)

• Mutation details: Sequence encoding FLP1 was adjoined to human ACTB promoter sequence, including 3 kb of promoter sequence and an 0.8 kb portion of exon 1. The promoter region drives expression in the germline. Recombinase activity is detected in a wide variety of tissues (spinal cord, heart, gonad, adrenal) as early as embryonic day 10.5. (J:78660)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	ACTB (human) Summary of all recombinase alleles driven by ACTB. Comparative matrix view of recombinase activities Alliance human ACTB gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 7 strains available Cell Lines: 0 lines available

Notes

The FLPe recombinase variant exhibits enhanced thermostability with recombination activity being 4X and 10X that of wildtype FLP at 37C and 40C, respectively. Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities.

References

• Original: J:78660 Rodriguez CI, et al., High-efficiency deleter mice show that FLPe is an alternative to Cre-loxP. Nat Genet. 2000 Jun;25(2):139-40
• All: 1116 reference(s)