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Targeted Allele Detail
Symbol: Car9tm1Spas
Name: carbonic anhydrase 9; targeted mutation 1, Silvia Pastorekova
MGI ID: MGI:2448129
Synonyms: CA IX KO, Car9-
Gene: Car9  Location: Chr4:43507026-43513729 bp, + strand  Genetic Position: Chr4, 23.05 cM
Morphological changes in the stomach and intestine of Car2n/Car2n and Car9tm1Spas/Car9tm1Spas mice

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:80329
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA PGK-neo cassette was inserted into exon 1 by homologous recombination. The insertion of neo downstream of the signal peptide deleted 14 nt (268-282) of the proteoglycan domain and caused a frameshift mutation. RT-PCR analysis of homozygous mutant mice identified a frame shifted transcript lacking 5' sequence encoding the signal peptide and most of the proteoglycan domain. Though the mutant transcript contained 77 nt of the neo cassette, a 3' portion of exon 1, and all of exons 2 through 11, protein was undetected by Western blot analysis of stomach tissue. (J:80329)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Car9 Mutation:  37 strains or lines available
Original:  J:80329 Gut MO, et al., Gastric hyperplasia in mice with targeted disruption of the carbonic anhydrase gene Car9. Gastroenterology. 2002 Dec;123(6):1889-903
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.19
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